Biofilm productivity and concomitant cell autolysis in a membrane bioreactor.
Identifieur interne : 000526 ( Main/Exploration ); précédent : 000525; suivant : 000527Biofilm productivity and concomitant cell autolysis in a membrane bioreactor.
Auteurs : S. Govender [Allemagne]Source :
- Biotechnology letters [ 1573-6776 ] ; 2011.
Descripteurs français
- KwdFr :
- Azote (métabolisme), Biofilms (croissance et développement), Bioréacteurs (microbiologie), Biotechnologie (méthodes), Carbone (métabolisme), Membranes (microbiologie), Milieux de culture (composition chimique), Peroxidases (métabolisme), Phanerochaete (croissance et développement), Phanerochaete (enzymologie).
- MESH :
- composition chimique : Milieux de culture.
- croissance et développement : Biofilms, Phanerochaete.
- enzymologie : Phanerochaete.
- microbiologie : Bioréacteurs, Membranes.
- métabolisme : Azote, Carbone, Peroxidases.
- méthodes : Biotechnologie.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Culture Media.
- chemical , metabolism : Carbon, Nitrogen, Peroxidases.
- enzymology : Phanerochaete.
- growth & development : Biofilms, Phanerochaete.
- methods : Biotechnology.
- microbiology : Bioreactors, Membranes.
Abstract
Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition used nutrients permeating from the lumen of the ultrafiltration membrane matrix established nutrient gradients across the immobilised biofilm with distinct primary, stationary and decline growth phases observed. Severe nutrient C and N limitation did not change the cyclic nature of enzyme production (MnP(max) = 189.5 U l(-1)) but did reduce the overall bioreactor efficiency from 32 to 22 U l(-1) day(-1). Stress induced secondary metabolism resulted in concomitant cell autolysis causing biomass loss and increased operational flux after 20 days in the 33 day bioreactor operation cycle.
DOI: 10.1007/s10529-010-0443-1
PubMed: 20972820
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Govender</name><affiliation wicri:level="4"><nlm:affiliation>Institut für Biochemie, Technische Universität Dresden, 01062 Dresden, Germany. selvan.govender@daad-alumni.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Biochemie, Technische Universität Dresden, 01062 Dresden</wicri:regionArea><wicri:noRegion>01062 Dresden</wicri:noRegion><orgName type="university">Université technique de Dresde</orgName><placeName><settlement type="city">Dresde</settlement><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Dresde</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20972820</idno><idno type="pmid">20972820</idno><idno type="doi">10.1007/s10529-010-0443-1</idno><idno type="wicri:Area/Main/Corpus">000527</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000527</idno><idno type="wicri:Area/Main/Curation">000527</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000527</idno><idno type="wicri:Area/Main/Exploration">000527</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biofilm productivity and concomitant cell autolysis in a membrane bioreactor.</title><author><name sortKey="Govender, S" sort="Govender, S" uniqKey="Govender S" first="S" last="Govender">S. Govender</name><affiliation wicri:level="4"><nlm:affiliation>Institut für Biochemie, Technische Universität Dresden, 01062 Dresden, Germany. selvan.govender@daad-alumni.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Biochemie, Technische Universität Dresden, 01062 Dresden</wicri:regionArea><wicri:noRegion>01062 Dresden</wicri:noRegion><orgName type="university">Université technique de Dresde</orgName><placeName><settlement type="city">Dresde</settlement><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Dresde</region></placeName></affiliation></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biofilms (growth & development)</term><term>Bioreactors (microbiology)</term><term>Biotechnology (methods)</term><term>Carbon (metabolism)</term><term>Culture Media (chemistry)</term><term>Membranes (microbiology)</term><term>Nitrogen (metabolism)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Azote (métabolisme)</term><term>Biofilms (croissance et développement)</term><term>Bioréacteurs (microbiologie)</term><term>Biotechnologie (méthodes)</term><term>Carbone (métabolisme)</term><term>Membranes (microbiologie)</term><term>Milieux de culture (composition chimique)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (croissance et développement)</term><term>Phanerochaete (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Nitrogen</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Biofilms</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Biofilms</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bioréacteurs</term><term>Membranes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bioreactors</term><term>Membranes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Carbone</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition used nutrients permeating from the lumen of the ultrafiltration membrane matrix established nutrient gradients across the immobilised biofilm with distinct primary, stationary and decline growth phases observed. Severe nutrient C and N limitation did not change the cyclic nature of enzyme production (MnP(max) = 189.5 U l(-1)) but did reduce the overall bioreactor efficiency from 32 to 22 U l(-1) day(-1). Stress induced secondary metabolism resulted in concomitant cell autolysis causing biomass loss and increased operational flux after 20 days in the 33 day bioreactor operation cycle.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20972820</PMID><DateCompleted><Year>2011</Year><Month>04</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Biofilm productivity and concomitant cell autolysis in a membrane bioreactor.</ArticleTitle><Pagination><MedlinePgn>263-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-010-0443-1</ELocationID><Abstract><AbstractText>Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition used nutrients permeating from the lumen of the ultrafiltration membrane matrix established nutrient gradients across the immobilised biofilm with distinct primary, stationary and decline growth phases observed. Severe nutrient C and N limitation did not change the cyclic nature of enzyme production (MnP(max) = 189.5 U l(-1)) but did reduce the overall bioreactor efficiency from 32 to 22 U l(-1) day(-1). Stress induced secondary metabolism resulted in concomitant cell autolysis causing biomass loss and increased operational flux after 20 days in the 33 day bioreactor operation cycle.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Govender</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institut für Biochemie, Technische Universität Dresden, 01062 Dresden, Germany. selvan.govender@daad-alumni.de</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>10</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018441" MajorTopicYN="N">Biofilms</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019149" MajorTopicYN="N">Bioreactors</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008566" MajorTopicYN="N">Membranes</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20972820</ArticleId><ArticleId IdType="doi">10.1007/s10529-010-0443-1</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>District de Dresde</li><li>Saxe (Land)</li></region><settlement><li>Dresde</li></settlement><orgName><li>Université technique de Dresde</li></orgName></list><tree><country name="Allemagne"><region name="Saxe (Land)"><name sortKey="Govender, S" sort="Govender, S" uniqKey="Govender S" first="S" last="Govender">S. 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